NFS 3083 Biology Enzyme Kinetics Questions

Want create site? With you can do it easy.

NFS 3083 Biology Enzyme Kinetics Questions

• Put your name at the top of this assignment
• Do NOT delete the “Instructions” and the “Rubric”,
• Type your answers after each question (or question part), and save your work.
 Make sure to provide thorough and specific answers to each question. Try to be concise, making sure to address all the KEY POINTS. However, if you feel the need to explain, feel free to do so (don’t assume things are implied), because you would rather get more points for explaining than losing out the key points in trying to be concise (don’t ramble).
 While you may discuss the questions with your classmates, you each should write the answers down in your own words. Plagiarism (including copying and pasting outside sources or previous submissions) will not be accepted and can result in an automatic zero!
 All the answers to this assignment should be found in your notes and/or textbook, however you may use outside sources as well (make sure to cite in APA format!)
 One points will be given for writing what your favorite thing about winter after your name at the top of this assignment
• Do NOT email your assignments; instead, use the “TurnItIn” as explained below to submit your completed assignment on Canvas.
• Upload/attach your final and completed document through the “TurnItIn” and click “Submit”.
 Note: “Save as Draft” will NOT submit your assignment to be graded; only you can see the saved draft!
 You are allowed unlimited attempts to submit. This means that you can resubmit your assignment for a multiple times (before the assignment deadline!) if you are not happy with your first submitted document and had revised your answers. Only the last submission will be graded.
 Absolutely no late assignments will be accepted.
 For more detailed instructions on how to use the “TurnItIn” click on this link: https://servicecenter.twu.edu/TDClient/KB/ArticleDet?ID=15550
Rubric:
• This assignment is 50 points total (5% of grade):
 5 points for correct grammar/spelling and FOLLOWING INSTRUCTIONS.
 The rest of the points are indicated next to each question below.

Don't use plagiarized sources. Get Your Custom Essay on
NFS 3083 Biology Enzyme Kinetics Questions
From $8/Page
Order Essay

Questions:
1. You recently were hired at a pharmaceutical company and are responsible for developing a new medication that targets the enzyme PAI-1. One of the main functions of PAI-1 is to inhibit the activation of plasmin from plasminogen (involved in breakdown of blood clots). Therefore, elevated PAI-1 is a risk factor for thrombosis and atherosclerosis. To design your novel medication, you first need to answer the following questions: (13pt)
a) What is enzyme kinetics? Why should we measure it? (2pt)

b) What are Km and Vmax? What do they tell us? (3pt)

c) How would pH and temperature affect enzyme kinetics? (2pt)

d) You want to make sure your drug is specific, which model of specificity should your follow, the lock and key model or induced fit model? Why? Make sure to describe both models (3pt)

e) Your coworker, mentions something about basing your design on a transition state analog, what is this? What is a transition state? Why would the analog be a good basis for your drug design? (3pt)

2. You presented your drug design for the PAI-1 medication to your boss, Dr. LeMieux. However, she would like a little bit more information regarding its method of inhibition. Specifically, she would like to know the answers to the following questions: (10pt)
a) What four factors influence enzymatic activity? (2pt)

NFS 3083 Biology Enzyme Kinetics Questions

NFS 3083 Biology Enzyme Kinetics Questions

b) What are the three types of reversible inhibition? Describe each of them (3pt)

c) What is allosteric inhibition? Provide an example of it. This example does not have to be a real-life one, you can use letters and numbers to prove your point. (2pts)

d) What is the difference between zymogens and isozymes? How are isozymes similar to each other and how are they different? (3pt)

3. It is commonly held that “a calorie is a calorie”, (i.e. that diets of equal caloric content will result in identical weight change independent of macronutrient composition). However, there is controversy among scientists if this belief violates the laws of thermodynamics (Ex: “metabolic advantage” of low-carbohydrate diets compared to isocaloric diets of different compositions). (9pt)
a) What are the first two laws of thermodynamics? Make sure to describe each of them (2pt)

b) What do the terms entropy, enthalpy and free energy mean? What do positive and negative changes in free energy indicate in terms of exergonic and endergonic reactions? (4pt)

c) Based on your answers above, do you agree that the phrase “a calorie is a calorie” breaks the laws of thermodynamics? Why or why not? You may use outside sources to support your answer, just make sure to cite them. (3pt)

4. Your classmate, Patty Pioneer, comes to you in a panic and tells you that she is so confused about protein folding, and asks you to help her. You agree and answer her questions below: (8pt)
a) What determines the 3D structure of a protein? (1pt)

b) What is the hydrophobic effect and why is it important for protein folding? (2pts)

c) Can you explain each of the different levels of protein structure? Patty is a visual learner, so make sure to include pictures with each description. (5pts) (You can hand draw this and post a picture here or use shapes in Powerpoint).

5. Complete the crossword (5pts). (You can put the answers right next to hints or in a numbered list if you like)

Across
2. amino acid Q
5. A _____ – ____ binds more tightly to the active site than the does the substrate molecule
6. level of protein structure that is three-dimensional structure of folding
8. amino acid E
11. amino acid W
12. amino acid D
14. interactions that drive folding
17. level of protein structure that is a-helix and b-sheets
18. complete enzyme including all of the protein subunits and prosthetic groups
20. an enzyme without its prosthetic groups

Down
1. loss of structure and function
3. describes a reaction for which the free-energy change (delta G) is negative
4. level of protein structure that is single chain of amino acids
7. the randomness of the components of a chemical system S
9. level of protein structure that is the assembly of individual polypeptides into a larger functional cluster
10. inhibitor that binds only to the ES complex, and therefore cannot bind to the substrate-binding site
13. The pH at which the numbers of positive and negative charges on an amino acid are equal is referred to as the ______
15. gives each amino acid their “identity”
16. trypsin is to trypsinogen as active enzyme is to
19. the energy of heat content of a system H

Did you find apk for android? You can find new and apps.

Calculate the price
Make an order in advance and get the best price
Pages (550 words)
$0.00
*Price with a welcome 20% discount applied.
Pro tip: If you want to save more money and pay the lowest price, you need to set a more extended deadline.
We know how difficult it is to be a student these days. That's why our prices are one of the most affordable on the market, and there are no hidden fees.

Instead, we offer bonuses, discounts, and free services to make your experience outstanding.
How it works
Receive a 100% original paper that will pass Turnitin from a top essay writing service
step 1
Upload your instructions
Fill out the order form and provide paper details. You can even attach screenshots or add additional instructions later. If something is not clear or missing, the writer will contact you for clarification.
Pro service tips
How to get the most out of your experience with Top Writers 4Me
One writer throughout the entire course
If you like the writer, you can hire them again. Just copy & paste their ID on the order form ("Preferred Writer's ID" field). This way, your vocabulary will be uniform, and the writer will be aware of your needs.
The same paper from different writers
You can order essay or any other work from two different writers to choose the best one or give another version to a friend. This can be done through the add-on "Same paper from another writer."
Copy of sources used by the writer
Our college essay writers work with ScienceDirect and other databases. They can send you articles or materials used in PDF or through screenshots. Just tick the "Copy of sources" field on the order form.
Testimonials
See why 10k+ students have chosen us as their sole writing assistance provider
Check out the latest reviews and opinions submitted by real customers worldwide and make an informed decision.
Microbiology
Perfect! Thank you guys for all of your great help! :)
Customer 452483, August 23rd, 2021
Healthcare Writing & Communications
Perfect! Thank you for also remembering to highlight the necessary words. Appreciate all of your help and hard work!
Customer 452483, November 7th, 2021
Mathematics
PERFECT! Thanks a million, you're all awesome!!!!
Customer 452483, July 7th, 2021
English 101
Perfect! Thank you so much for all of your hard work and help! Appreciate it!!
Customer 452483, August 30th, 2021
Psychology
Thank you so much for all of your hard work & help! It’s perfect! Appreciate it!!
Customer 452483, September 11th, 2021
Healthcare Writing & Communications
Perfect! Thank you so much for all of your help!
Customer 452483, October 31st, 2021
Healthcare Writing & Communications
Thank you so much for all of your hard work! Appreciate it all!
Customer 452483, November 14th, 2021
Nursing
Good job. Thank you
Customer 452487, September 16th, 2021
Education
Great good
Customer 452555, February 14th, 2022
Introduction to Sociology
Thank you very much for all of your hard work!
Customer 452483, October 15th, 2021
Nursing
Good job! Thank you
Customer 452487, October 17th, 2021
Psychology
Thank you for all of your help and hard work, truly appreciate you guys always being there with my hectic schedule. Thank you again!
Customer 452483, September 20th, 2021
1159
Customer reviews in total
96%
Current satisfaction rate
2 pages
Average paper length
47%
Customers referred by a friend
OUR GIFT TO YOU
20% OFF your first order
Use a coupon TOP20 and enjoy expert help with any task at the most affordable price.
Claim my 20% OFF Order in Chat
Open chat
Chat with us Live Here
Get in Touch with Our Live Chat Agents via WhatsApp on +1(346) 416-0954. Get Unlimited Access to Answers on Any Queries With Your Paper.