RFLPs, VNTR, SNP – definitions and use
What are restriction fragment length polymorphisms (RFLPs), variable number tandem repeat (VNTRs), and single nucleotide polymorphisms (SNPs)? What is the relationship among these? How do we use this technology to, for example, identify paternity? Solution Preview
RFLP stands for restriction fragment length polymorphism. This technique relies on the fact that, when digested by restriction enzymes, a pattern of DNA fragments will be generated from the DNA of the individual that is unique due to the unique sequence of their DNA. By examining the banding pattern of this DNA run on a gel, the identity of particular alleles can be identified by the banding pattern.
VNTR stands for variable number tandem repeats. Analysis of VNTRs relies on the fact that there are regions on many chromosomes where there are …
This solution explains what is meant by restriction fragment length polymorphism (RFLP), variable number tandem repeat (VNTR), and single nucleotide polymorphism (SNP) analysis. It also details the relationships between them and how they are used.
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